BIOSYNTHESIS OF COPPER OXIDE NANOPARTICLES AND EVALUATION OF THEIR ANTIMICROBIAL PROPERTIES

Objective: This research was carried out to synthesize and characterize copper oxide nanoparticles (CuONPs) using Vernonia amygdalina leaf extract investigate the in vitro antimicrobial properties clinical microbial isolates. Methods: The CuONPs were synthesized by heating a mixture of sulfate pentahydrate V. aqueous extract. characterized Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), particle size analysis. Phytochemical analysis determine bio-molecules that served as reducing agent during synthesis CuONPs. activities evaluated agar disc diffusion method against Staphylococcus aureus, Escherichia coli, Candida albicans. Ampicillin fluconazole used reference antibacterial antifungal agents, respectively. Results: nanometer dimension exhibited significant activity (P<0.05) tested microbes. However, standard drug, ampicillin, showed higher S. aureus E. coli with inhibition zone diameter (IZD) 13.10±0.38 mm 11.80±0.12 mm, Fluconazole had no C. albicans while demonstrated good but lacked combination plant an IZD 10.37±0.72 mm. Conclusion: An eco-friendly, simple, reproducible, economical have been findings indicate could be agent.